Nondestructive Detection and Quantification of Blueberry Bruising using Near-infrared (NIR) Hyperspectral Reflectance Imaging

Currently, blueberry bruising is evaluated by either human visual/tactile inspection or firmness measurement instruments. These methods are destructive, time-consuming, and subjective. The goal of this paper was to develop a non-destructive approach for blueberry bruising detection and quantification. Experiments were conducted on 300 samples of southern highbush blueberry (Camellia, Rebel, and Star) and on 1500 samples of northern highbush blueberry (Bluecrop, Jersey, and Liberty) for hyperspectral imaging analysis, firmness measurement, and human evaluation. An algorithm was developed to automatically calculate a bruise ratio index (ratio of bruised to whole fruit area) for bruise quantification. The spectra of bruised and healthy tissues were statistically separated and the separation was independent of cultivars. Support vector machine (SVM) classification of the spectra from the regions of interest (ROIs) achieved over 94%, 92%, and 96% accuracy on the training set, independent testing set, and combined set, respectively. The statistical results showed that the bruise ratio index was equivalent to the measured firmness but better than the predicted firmness in regard to effectiveness of bruise quantification, and the bruise ratio index had a strong correlation with human assessment (R-squared = 0.78 − 0.83). Therefore, the proposed approach and the bruise ratio index are effective to non-destructively detect and quantify blueberry bruising.

Detailed information refers to our paper Jiang, Y., C. Li, and F. Takeda. 2016. Nondestructive detection and quantification of blueberry bruising using near-infrared (NIR) hyperspectral reflectance imaging. Scientific Reports. 6: srep35679.

Figure 1. Flowchart of the hyperspectral image processing from flat field correction to calculation of the bruise ratio index.

Figure 2. Mean spectra (solid line) and standard deviation (error bar) of healthy and bruised tissue.

Figure 3. Grayscale image at 1200 nm, classified image, and color image of sliced fruit of representative results from southern highbush cultivars.

Figure 4. Grayscale image at 1200 nm, classified image, and color image of sliced fruit of representative results from northern highbush cultivars.